Vejleder: Stefan Vogel
Forudsætninger: KE505 og KE506 skal være bestået.
Projektbeskrivelse
Introduction: Chemically engineered and functionalized nanoscale compartments are used in bottom-up synthetic biology to construct compartmentalized chemical processes. Here, we use DNA-encoded (barcoded) nanoreactor design for successive fusion of multiple nanoreactors (lipid nanoreactors). In contrast to fusion of “compartments” found in nature this artificial fusion cascade exploits the versatile encoding-potential of DNA based on DNA hybridization and is generally applicable to nanoreactors of different size. The methods allow combinatorial/parallelized chemistries in very small volumes [1-2]
Figure 1. Schematic representation of a three-stage cascade of liposome fusion encoded by three sets of complementary LiNAs (lipid modified nucleic acids). Content mixing are the consequence of the zipper-like hybridization of membrane-anchored LiNAs (left insert).
Methods: We look forward to methods in supramolecular chemistry such as assembly of functional nanoparticles in mind. This project is designed for students interested to learn more supramolecular chemistry combined with organic synthetic chemistry with applications in catalysis, parallel and combinatorial chemistry. The project is designed to introduce supramolecular chemistry in combination with functional nanoreactors which are designed to help producing libraries of compounds with medicinal properties and potential bioactivity in mind.
References: [1] Tian X., Risgaard N. A., Löffler P. M. G., Vogel S.*, DNA-Programmed Lipid Nanoreactors for Synthesis of Carbohydrate Mimetics by Fusion of Aqueous Sub-attoliter Compartments, J. Am. Chem. Soc. 2023, 145, 36, 19633–19641. [2] P. M. G. Löffler, O. Ries, A. Rabe, A. H. Okholm, R. P. Thomsen, J. Kjems and S. Vogel, Angew. Chem., Int. Ed., 2017, 56, 13228–13231.