ECTS: 15
Faglige nøgleord: Permeation, Ocular biological barriers, 3Rs, Artificial membranes.
Antal Studerende: 1-2
Vejledere: René Holm og Zahra Ghaemmaghamian (medvejleder)
Beskrivelse: The 3Rs refer to Replacement, Reduction, and Refinement, which are principles aimed at ensuring the rational and respectful use of laboratory animals in biological and biomedical research. Different approaches could be used to apply 3Rs in preclinical experimentation, such as using artificial membranes in permeability study of the drug compounds. Rabbit models are mainly being used for ocular drug development studies. If artificial membranes to any extend could mimic the animal models they would be valuable tools in aiming 3Rs [1].
PermeaPad® is a fully artificial phospholipid-based biomimetic membrane with a layered structure composed of soybean phosphatidylcholine S-100 deposited between two support sheets. PermeaPad® is available in several ready-to-use formats, including disks mounted in side-by-side diffusion cells or Franz diffusion cells. It has been observed to have a good linear correlation between permeability coefficients obtained with PermeaPad® and the Caco-2 cell assay or Parallel Artificial Membrane Permeability Assay (PAMPA) from literature [2].
The aim of this project is to assess the possibility of mimicking animal-based models with in vitro models via PermeaPad® to study ocular biological barriers [3].
Metoder: HPLC, UV-visible spectroscopy, Franz diffusion cell.
Referencer:
[1] A. G. Rodriguez, "Strategies to apply 3Rs in preclinical testing," Pharmacol Res Perspect, vol. 9:e00863, 2021.
[2] Philippe Berben, "Drug permeability profiling using cell-free permeation tools: Overview and applications," European Journal of Pharmaceutical Sciences, vol. 119, p. 219–233, 2018.
[3] Q. Bao, "In Vitro and Ex Vivo Correlation of Drug Release from Ophthalmic Ointments," J Control Release., vol. 28, no. 276, p. 93–101, 2018 April .