Vejleder: Stefan Vogel
Forudsætninger: KE505 og KE506 skal være bestået.
Projektbeskrivelse
Baggrund for projektet: The characterization of nanoparticles has become an important task in modern chemical and pharmaceutical research, and in particular drug delivery. Especially the increasing success of biologicals as drugs has demanded advanced formulation technology which often involves lipid-based nano- and microparticles. To characterize nanoparticle stability over time, particle size, size dispersity and surface charge at same time require user friendly advanced instrumentation.
Figure 1. ) Schematic illustration of key components for G4-decoy ON delivery systems 85 nm sized POPC liposomes with membrane anchored G4-ON and a cell-penetrating peptide (TAT) attached.1 (B) Extracellular vesicle (e.g., exosome) as example for a biological lipid nanoparticle.2
Problemformulering: Which parameters effect lipid nanoparticle size, evaluation of different lipid mixture, surface charge, stability over time? How are lipid nanoparticles routinely characterized?
Methoder: We look forward to students interested to apply methods from spectroscopy, biophysics and chemistry to current lipid nanoparticle formulation. This project is designed for students interested to learn more about lipid nanoparticles with applications in drug delivery and modern formulation of therapeutic peptides and oligonucleotides.
Vejledning: Biweekly meetings that include data presentation and interpretations as well as time for discussions.
Referencer:
[1] Cogoi, S., Jakobsen, U., Pedersen, E. B., Vogel S.* & Xodo, L. E.,* Lipid-modified G4-decoy oligonucleotide anchored to nanoparticles: delivery and bioactivity in pancreatic cancer cells Sci. Rep. 2016, 6, 1-13.
[2] Bakowski, K. and Vogel, S.*, Evolution of complexity in non-viral oligonucleotide delivery systems, RNA Biology, 2022, 19(1), pp. 1256–1275.